cDNA Synthesis

cDNA synthesis (complementary DNA synthesis) is a fundamental molecular biology technique that converts RNA into complementary DNA using the enzyme reverse transcriptase. This process enables researchers to analyze gene expression, study mRNA transcripts, and perform quantitative PCR (qPCR), RT-PCR, RNA sequencing (RNA-seq), and transcriptome analysis. cDNA is more stable than RNA and serves as a reliable template for cloning, genetic analysis, and biotechnology research.